PCR and Real Time PCR
Let’s fight back against genetic disorders with precision and personalized treatment.
PCR and Real Time PCR
The power of genetic testing lies in the ability to precisely detect and amplify specific DNA or RNA sequences. That’s where PCR (Polymerase Chain Reaction) and RT-PCR (Reverse Transcriptase PCR) come in as game-changers. PCR uses a series of heating and cooling cycles to amplify DNA, whereas RT-PCR deals with cDNA, which has completely revolutionized genetic testing by allowing rapid and precise detection of genetic material from various sources. Additionally, advanced PCR techniques, such as ARMS, RFLP, SSCP, and SSOP, make PCR and RT-PCR even more versatile. These techniques enable highly sensitive and specific detection of genetic variations associated with diseases, making them powerful tools in the fight against genetic disorders. From identifying single nucleotide polymorphisms with ARMS to distinguishing DNA fragments of different lengths with RFLP, these advanced techniques have brought new levels of accuracy and sensitivity to genetic testing. PCR and RT-PCR technologies, coupled with advanced techniques, are paving the way for personalized medicine and precision treatment, providing hope for millions of people. Real Time PCR is used to amplify and detect specific DNA sequences in real-time as the reaction progresses. The key feature of real-time PCR is the use of fluorescent dyes/probes that bind to amplified DNA during the PCR reaction. Real-time PCR multiplexing with different dyes enables researchers to detect, quantify, and analyze specific DNA sequences in real time. This method has a wider application and is a powerful tool in molecular biology and genetics.
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